Journal Article

Stereological estimation of cell wall density of DR12 tomato mutant using three-dimensional confocal imaging

David Legland, Fabienne Guillon, Kiên Kiêu, Brigitte Bouchet and Marie-Françoise Devaux

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 105, issue 2, pages 265-276
Published in print February 2010 | ISSN: 0305-7364
Published online December 2009 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcp283
Stereological estimation of cell wall density of DR12 tomato mutant using three-dimensional confocal imaging

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  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

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Background and Aims

The cellular structure of fleshy fruits is of interest to study fruit shape, size, mechanical behaviour or sensory texture. The cellular structure is usually not observed in the whole fruit but, instead, in a sample of limited size and volume. It is therefore difficult to extend measurements to the whole fruit and/or to a specific genotype, or to describe the cellular structure heterogeneity within the fruit.

Methods

An integrated method is presented to describe the cellular structure of the whole fruit from partial three-dimensional (3D) observations, involving the following steps: (1) fruit sampling, (2) 3D image acquisition and processing and (3) measurement and estimation of relevant 3D morphological parameters. This method was applied to characterize DR12 mutant and wild-type tomatoes (Solanum lycopersicum).

Key Results

The cellular structure was described using the total volume of the pericarp, the surface area of the cell walls and the ratio of cell-wall surface area to pericarp volume, referred to as the cell-wall surface density. The heterogeneity of cellular structure within the fruit was investigated by estimating variations in the cell-wall surface density with distance to the epidermis.

Conclusions

The DR12 mutant presents a greater pericarp volume and an increase of cell-wall surface density under the epidermis.

Keywords: Stereology; 3D morphology estimation; tomato fruit; cellular structure; image analysis; confocal microscopy

Journal Article.  8362 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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