Journal Article

Analysis of <i>Arabidopsis thaliana</i> root growth kinetics with high temporal and spatial resolution

Nima Yazdanbakhsh and Joachim Fisahn

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 105, issue 5, pages 783-791
Published in print May 2010 | ISSN: 0305-7364
Published online May 2010 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcq048
Analysis of Arabidopsis thaliana root growth kinetics with high temporal and spatial resolution

More Like This

Show all results sharing these subjects:

  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Background

Methods exist to quantify the distribution of growth rate over the root axis. However, non-destructive, high-throughput evaluations of total root elongation in controlled environments and the field are lacking in growth studies. A new imaging approach to analyse total root elongation is described.

Scope

High pixel resolution of the images enables the study of growth in short time intervals and provides high temporal resolution. Using the method described, total root elongation rates are calculated from the displacement of the root tip. Although the absolute root elongation rate changes in response to growth conditions, this set-up enables root growth of Arabidopsis wild-type seedlings to be followed for more than 1 month after germination. The method provides an easy approach to decipher root extension rate and much simpler calculations compared with other methods that use segmental growth to address this question.

Conclusions

The high temporal resolution allows small modifications of total root elongation growth to be revealed. Furthermore, with the options to investigate growth of various mutants in diverse growth conditions the present tool allows modulations in root growth kinetics due to different biotic and abiotic stimuli to be unravelled. Measurements performed on Arabidopsis thaliana wild-type (Col0) plants revealed rhythms superimposed on root elongation. Results obtained from the starchless mutant pgm, however, present a clearly modified pattern. As expected, deviation is strongest during the dark period.

Keywords: Arabidopsis thaliana; image processing; non invasive monitoring; pgm; root phenotyping; total root elongation

Journal Article.  5580 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.