Journal Article

Totomatix: a novel automatic set-up to control diurnal, diel and long-term plant nitrate nutrition

Stéphane Adamowicz, Jacques Le Bot, Ruth Huanosto Magaña and José Fabre

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 109, issue 1, pages 309-319
Published in print January 2012 | ISSN: 0305-7364
Published online October 2011 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcr254
Totomatix: a novel automatic set-up to control diurnal, diel and long-term plant nitrate nutrition

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  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

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Background

Stand-alone nutritional set-ups are useful tools to grow plants at defined nutrient availabilities and to measure nutrient uptake rates continuously, in particular that for nitrate. Their use is essential when the measurements are meant to cover long time periods. These complex systems have, however, important drawbacks, including poor long-term reliability and low precision at high nitrate concentration. This explains why the information dealing with diel dynamics of nitrate uptake rate is scarce and concerns mainly young plants grown at low nitrate concentration.

Scope

The novel system detailed in this paper has been developed to allow versatile use in growth rooms, greenhouses or open fields at nitrate concentrations ranging from a few micro- to several millimoles per litres. The system controls, at set frequencies, the solution nitrate concentration, pH and volumes. Nitrate concentration is measured by spectral deconvolution of UV spectra. The main advantages of the set-up are its low maintenance (weekly basis), an ability to diagnose interference or erroneous analyses and high precision of nitrate concentration measurements (0·025 % at 3 mm). The paper details the precision of diurnal nitrate uptake rate measurements, which reveals sensitivity to solution volume at low nitrate concentration, whereas at high concentration, it is mostly sensitive to the precision of volume estimates.

Conclusions

This novel set-up allows us to measure and characterize the dynamics of plant nitrate nutrition at high temporal resolution (minutes to hours) over long-term experiments (up to 1 year). It is reliable and also offers a novel method to regulate up to seven N treatments by adjusting the daily uptake of test plants relative to controls, in variable environments such as open fields and glasshouses.

Keywords: Automatic set-up; nitrogen; nitrate; nutrition; uptake rate; diel; diurnal; hydroponics; UV spectrophotometry; deconvolution

Journal Article.  7320 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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