Journal Article

Induced polyploidy dramatically increases the size and alters the shape of fruit in <i>Actinidia chinensis</i>

Jin-Hu Wu, A. Ross Ferguson, Brian G. Murray, Yilin Jia, Paul M. Datson and Jingli Zhang

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 109, issue 1, pages 169-179
Published in print January 2012 | ISSN: 0305-7364
Published online October 2011 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcr256
Induced polyploidy dramatically increases the size and alters the shape of fruit in Actinidia chinensis

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  • Ecology and Conservation
  • Evolutionary Biology
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Background and Aims

Some otherwise promising selections of Actinidia chinensis (kiwifruit) have fruit that are too small for successful commercialization. We have therefore made the first detailed study in diploid kiwifruit of the effects of chromosome doubling induced by colchicine on fruit size, shape and crop loading.

Methods

Flow cytometric analysis of young leaves and chromosome analysis of flower buds and root tips was used to confirm the stability of induced autotetraploids. Fruit weight, size and crop load were measured in the third year after planting in the field and for three consecutive years. DNA fingerprinting was used to confirm the origin of the material.

Key Results

There was a very significant increase in fruit size in induced autotetraploids of different genotypes of A. chinensis. With the commercially important diploid cultivar ‘Hort16A’, most regenerants, Type A plants, had fruit which were much the same shape as fruit of the diploid but, at the same fruit load, were much larger and heavier. Some regenerants, Type B plants, produced fruit similar to ‘fasciated’ fruit. Fruit of the autotetraploids induced from three female red-fleshed A. chinensis selections were also 50–60 % larger than fruit of their diploid progenitors. The main increase in fruit dimensions was in their diameters. These improved fruit characteristics were stable over several seasons.

Conclusions

Chromosome doubling has been shown to increase significantly fruit size in autotetraploid A. chinensis, highlighting the considerable potential of this technique to produce new cultivars with fruit of adequate size. Other variants with differently shaped fruit were also produced but the genetic basis of this variation remains to be elucidated. Autoploids of other Actinidia species with commercial potential may also show improved fruit characteristics, opening up many new possibilities for commercial development.

Keywords: Actinidia chinensis; autotetraploid; chromosome doubling; chromosome number; colchicine; DNA fingerprinting; flow cytometry; fruit shape; fruit size; kiwifruit; red-fleshed kiwifruit; somaclonal variation

Journal Article.  6298 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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