Journal Article

Hybrid proline-rich proteins: novel players in plant cell elongation?

Lenka Dvořáková, Miroslav Srba, Zdenek Opatrny and Lukas Fischer

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 109, issue 2, pages 453-462
Published in print February 2012 | ISSN: 0305-7364
Published online October 2011 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcr278
Hybrid proline-rich proteins: novel players in plant cell elongation?

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  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

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Background and Aims

Hybrid proline-rich proteins (HyPRPs) represent a large family of putative cell-wall proteins characterized by the presence of a variable N-terminal domain and a conserved C-terminal domain that is related to non-specific lipid transfer proteins. The function of HyPRPs remains unclear, but their widespread occurrence and abundant expression patterns indicate that they may be involved in a basic cellular process.

Methods

To elucidate the cellular function of HyPRPs, we modulated the expression of three HyPRP genes in tobacco (Nicotiana tabacum) BY-2 cell lines and in potato (Solanum tuberosum) plants.

Key Results

In BY-2 lines, over-expression of the three HyPRP genes with different types of N-terminal domains resulted in similar phenotypic changes, namely increased cell elongation, both in suspension culture and on solid media where the over-expression resulted in enhanced calli size. The over-expressing cells showed increased plasmolysis in a hypertonic mannitol solution and accelerated rate of protoplast release, suggesting loosening of the cell walls. In contrast to BY-2 lines, no phenotypic changes were observed in potato plants over-expressing the same or analogous HyPRP genes, presumably due to more complex compensatory mechanisms in planta.

Conclusions

Based on the results from BY-2 lines, we propose that HyPRPs, more specifically their C-terminal domains, represent a novel group of proteins involved in cell expansion.

Keywords: Cell extension; cell wall loosening; cell wall protein; hybrid proline-rich proteins; HyPRP; lipid transfer proteins; LTP; potato; Solanum tuberosum; tobacco; Nicotiana tabacum; BY-2 cell line

Journal Article.  6820 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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