Journal Article

Spatiotemporal variation of leaf epidermal cell growth: a quantitative analysis of <i>Arabidopsis thaliana</i> wild-type and triple <i>cyclinD3</i> mutant plants

Joanna Elsner, Marek Michalski and Dorota Kwiatkowska

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 109, issue 5, pages 897-910
Published in print April 2012 | ISSN: 0305-7364
Published online February 2012 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcs005
Spatiotemporal variation of leaf epidermal cell growth: a quantitative analysis of Arabidopsis thaliana wild-type and triple cyclinD3 mutant plants

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  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

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Background and Aims

The epidermis of an expanding dicot leaf is a mosaic of cells differing in identity, size and differentiation stage. Here hypotheses are tested that in such a cell mosaic growth is heterogeneous and changes with time, and that this heterogeneity is not dependent on the cell cycle regulation per se.

Methods

Shape, size and growth of individual cells were followed with the aid of sequential replicas in expanding leaves of wild-type Arabidopsis thaliana and triple cyclinD3 mutant plants, and combined with ploidy estimation using epi-fluorescence microscopy.

Key Results

Relative growth rates in area of individual epidermal cells or small cell groups differ several fold from those of adjacent cells, and change in time. This spatial and temporal variation is not related to the size of either the cell or the nucleus. Shape changes and growth within an individual cell are also heterogeneous: anticlinal wall waviness appears at different times in different wall portions; portions of the cell periphery in contact with different neighbours grow with different rates. This variation is not related to cell growth anisotropy. The heterogeneity is typical for both the wild type and cycD3.

Conclusions

Growth of leaf epidermis exhibits spatiotemporal variability.

Keywords: Leaf epidermis; cell mosaic; cell expansion; growth heterogeneity; variation at cellular and sub-cellular levels; Arabidopsis thaliana; cyclinD3

Journal Article.  8637 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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