Journal Article

Congratulations, you have been carefully chosen to represent an important developmental regulator!

Alon Samach

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 111, issue 3, pages 329-333
Published in print March 2013 | ISSN: 0305-7364
Published online July 2012 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcs161
Congratulations, you have been carefully chosen to represent an important developmental regulator!

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  • Ecology and Conservation
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Background

Studying a process in a new species often relies on focusing our attention to a candidate gene, encoding a protein similar to one with a known function. Not all the choices seem to be prudent.

Scope

This Viewpoint includes an overview of issues that are encountered during research of candidate genes. Defining a match for a gene of interest, deciding whether variation in ESTs or RNAseq data for a certain transcript, represent more than one gene. The problem of incorrect annotation of genes due to incorrect in-silico splicing, is also mentioned. The author's humble opinion on how to deal with these issues is provided.

Conclusions

The vast amount of new sequence data provides us with great possibilities for giant leaps in our understanding. Still, we cannot afford to skip over the tedious steps required to confirm that we are indeed studying the correct gene, and try to be sure that the complex expression pattern we observe is not a composite of several genes.

Keywords: Homology; orthology; MADS box; FLOWERING LOCUS T; FLC; candidate gene; citrus; Citrus clementina; apple; Malus domestica

Journal Article.  3306 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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