Journal Article

<i>At3g08030</i> transcript: a molecular marker of seed ageing

Luz Elena Garza-Caligaris, Aida Odette Avendaño-Vázquez, Sandra Alvarado-López, Esther Zúñiga-Sánchez, Alma Orozco-Segovia, Rigoberto V. Pérez-Ruíz and Alicia Gamboa-deBuen

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 110, issue 6, pages 1253-1260
Published in print November 2012 | ISSN: 0305-7364
Published online September 2012 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcs200
At3g08030 transcript: a molecular marker of seed ageing

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  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

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Background and Aims

Prolonged storage generally reduces seed viability and vigour, although the rate of deterioration varies among species and environmental conditions. Here, we suggest a possible ageing molecular marker: At3g08030 mRNA. At3g08030 is a member of the DUF642 highly conserved family of cell-wall-associated proteins that is specific for spermatophytes.

Methods

At3g08030 expression was performed by RT-PCR and qRT-PCR analysis in seed samples differing in their rate of germination and final germination following a matrix priming and/or controlled deterioration (rapid ageing) treatment.

Key Results

The At3g08030 gene transcript was present during the entire Arabidopsis thaliana plant life cycle and in seeds, during maturation, the ripening period and after germination. Matrix priming treatment increased the rate of germination of control seeds and seeds aged by controlled deterioration. Priming treatments also increased At3g08030 expression. To determine whether the orthologues of this gene are also age markers in other plant species, At3g08030 was cloned in two wild species, Ceiba aesculifolia and Wigandia urens. As in A. thaliana, the At3g08030 transcript was not present in aged seeds of the tested species but was present in recently shed seeds. A reduction in germination performance of the aged seeds under salt stress was determined by germination assays.

Conclusions

At3g08030 mRNA detection in a dry seed lot has potential for use as a molecular marker for germination performance in a variety of plant species.

Keywords: Seed ageing; molecular marker; At3g08030; DUF642 gene family; Arabidopsis thaliana; Ceiba aesculifolia; Wigandia urens

Journal Article.  4908 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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