Journal Article

Gene dosage effect of WEE1 on growth and morphogenesis from arabidopsis hypocotyl explants

Natasha Spadafora, Lara Perrotta, Jeroen Nieuwland, Diego Albani, M. Beatrice Bitonti, Robert J. Herbert, John H. Doonan, Angela M. Marchbank, Ilario Siciliano, Anne Lentz Grønlund, Dennis Francis and Hilary J. Rogers

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 110, issue 8, pages 1631-1639
Published in print December 2012 | ISSN: 0305-7364
Published online October 2012 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcs223
Gene dosage effect of WEE1 on growth and morphogenesis from arabidopsis hypocotyl explants

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  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

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Background and Aims

How plant cell-cycle genes interface with development is unclear. Preliminary evidence from our laboratory suggested that over-expression of the cell cycle checkpoint gene, WEE1, repressed growth and development. Here the hypothesis is tested that the level of WEE1 has a dosage effect on growth and development in Arabidospis thaliana. To do this, a comparison was made of the development of gain- and loss-of-function WEE1 arabidopsis lines both in vivo and in vitro.

Methods

Hypocotyl explants from an over-expressing Arath;WEE1 line (WEE1oe), two T-DNA insertion lines (wee1-1 and wee1-4) and wild type (WT) were cultured on two-way combinations of kinetin and naphthyl acetic acid. Root growth and meristematic cell size were also examined.

Key Results

Quantitative data indicated a repressive effect in WEE1oe and a significant increase in morphogenetic capacity in the two T-DNA insertion lines compared with WT. Compared with WT, WEE1oe seedlings exhibited a slower cell-doubling time in the root apical meristem and a shortened primary root, with fewer laterals, whereas there were no consistent differences in the insertion lines compared with WT. However, significantly fewer adventitious roots were recorded for WEE1oe and significantly more for the insertion mutant wee1-1. Compared with WT there was a significant increase in meristem cell size in WEE1oe for all three ground tissues but for wee1-1 only cortical cell size was reduced.

Conclusions

There is a gene dosage effect of WEE1 on morphogenesis from hypocotyls both in vitro and in vivo.

Keywords: Arabidopsis thaliana; cell cycle; development; growth; hypocotyl; tissue culture; WEE1

Journal Article.  5224 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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