Journal Article

Immunolocalization of arabinogalactan proteins (AGPs) in reproductive structures of an early-divergent angiosperm, <i>Trithuria</i> (Hydatellaceae)

Mário Costa, Ana Marta Pereira, Paula J. Rudall and Sílvia Coimbra

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 111, issue 2, pages 183-190
Published in print February 2013 | ISSN: 0305-7364
Published online November 2012 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcs256
Immunolocalization of arabinogalactan proteins (AGPs) in reproductive structures of an early-divergent angiosperm, Trithuria (Hydatellaceae)

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Background and Aims Trithuria

is the sole genus of Hydatellaceae, a family of the early-divergent angiosperm lineage Nymphaeales (water-lilies). In this study different arabinogalactan protein (AGP) epitopes in T. submersa were evaluated in order to understand the diversity of these proteins and their functions in flowering plants.

Methods

Immunolabelling of different AGPs and pectin epitopes in reproductive structures of T. submersa at the stage of early seed development was achieved by immunofluorescence of specific antibodies.

Key Results

AGPs in Trithuria pistil tissues could be important as structural proteins and also as possible signalling molecules. Intense labelling was obtained with anti-AGP antibodies both in the anthers and in the intine wall, the latter associated with pollen tube emergence.

Conclusions

AGPs could play a significant role in Trithuria reproduction, due to their specific presence in the pollen tube pathway. The results agree with labellings obtained for Arabidopsis and confirms the importance of AGPs in angiosperm reproductive structures as essential structural components and probably important signalling molecules.

Keywords: Trithuria; Hydatellaceae; arabinogalactan proteins; monoclonal antibodies; reproductive units; starch grains

Journal Article.  4001 words.  Illustrated.

Subjects: Plant Reproduction and Propagation

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