Journal Article

Proteomics in <i>Drosophila melanogaster</i>

Kathryn S. Lilley and Delia R. Griffiths

in Briefings in Functional Genomics

Volume 2, issue 2, pages 106-113
Published in print July 2003 | ISSN: 2041-2649
Published online July 2003 | e-ISSN: 2041-2647 | DOI: https://dx.doi.org/10.1093/bfgp/2.2.106
Proteomics in Drosophila melanogaster

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To be able to understand cellular mechanisms, we require fully integrated data sets combining information about gene expression, protein expression, post-translational modification states, sub-cellular location and complex formation. Proteomics is a very powerful technique that can be applied to interrogate changes at the protein level. Studying this effectively requires specialised facilities within research institutes. Here, we describe the setting up and operation of such a facility, providing a resource for the Arabidopsis and Drosophila research communities.

Keywords: Proteomics; Drosophila melanogaster; LC-MS/MS; two-dimensional polyacrylamide gel electrophoresis; difference gel electrophoresis; isotope coded affinity tagging

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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