Journal Article

Physical protein–protein interactions predicted from microarrays

Ta-tsen Soong, Kazimierz O. Wrzeszczynski and Burkhard Rost

in Bioinformatics

Volume 24, issue 22, pages 2608-2614
Published in print November 2008 | ISSN: 1367-4803
Published online October 2008 | e-ISSN: 1460-2059 | DOI: http://dx.doi.org/10.1093/bioinformatics/btn498

More Like This

Show all results sharing this subject:

  • Bioinformatics and Computational Biology

GO

Show Summary Details

Preview

Motivation: Microarray expression data reveal functionally associated proteins. However, most proteins that are associated are not actually in direct physical contact. Predicting physical interactions directly from microarrays is both a challenging and important task that we addressed by developing a novel machine learning method optimized for this task.

Results: We validated our support vector machine-based method on several independent datasets. At the same levels of accuracy, our method recovered more experimentally observed physical interactions than a conventional correlation-based approach. Pairs predicted by our method to very likely interact were close in the overall network of interaction, suggesting our method as an aid for functional annotation. We applied the method to predict interactions in yeast (Saccharomyces cerevisiae). A Gene Ontology function annotation analysis and literature search revealed several probable and novel predictions worthy of future experimental validation. We therefore hope our new method will improve the annotation of interactions as one component of multi-source integrated systems.

Contact: ts2186@columbia.edu

Supplementary information: Supplementary data are available at Bioinformatics online.

Journal Article.  5834 words.  Illustrated.

Subjects: Bioinformatics and Computational Biology

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.