Journal Article

Serial dilution curve: a new method for analysis of reverse phase protein array data

Li Zhang, Qingyi Wei, Li Mao, Wenbin Liu, Gordon B. Mills and Kevin Coombes

in Bioinformatics

Volume 25, issue 5, pages 650-654
Published in print March 2009 | ISSN: 1367-4803
Published online January 2009 | e-ISSN: 1460-2059 | DOI:

More Like This

Show all results sharing this subject:

  • Bioinformatics and Computational Biology


Show Summary Details


Reverse phase protein arrays (RPPAs) are a powerful high-throughput tool for measuring protein concentrations in a large number of samples. In RPPA technology, the original samples are often diluted successively multiple times, forming dilution series to extend the dynamic range of the measurements and to increase confidence in quantitation. An RPPA experiment is equivalent to running multiple ELISA assays concurrently except that there is usually no known protein concentration from which one can construct a standard response curve. Here, we describe a new method called ‘serial dilution curve for RPPA data analysis’. Compared with the existing methods, the new method has the advantage of using fewer parameters and offering a simple way of visualizing the raw data. We showed how the method can be used to examine data quality and to obtain robust quantification of protein concentrations.

Availability: A computer program in R for using serial dilution curve for RPPA data analysis is freely available at


Journal Article.  3235 words.  Illustrated.

Subjects: Bioinformatics and Computational Biology

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.