Journal Article

RNAither, an automated pipeline for the statistical analysis of high-throughput RNAi screens

Nora Rieber, Bettina Knapp, Roland Eils and Lars Kaderali

in Bioinformatics

Volume 25, issue 5, pages 678-679
Published in print March 2009 | ISSN: 1367-4803
Published online January 2009 | e-ISSN: 1460-2059 | DOI: http://dx.doi.org/10.1093/bioinformatics/btp014
RNAither, an automated pipeline for the statistical analysis of high-throughput RNAi screens

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Summary: We present RNAither, a package for the free statistical environment R which performs an analysis of high-throughput RNA interference (RNAi) knock-down experiments, generating lists of relevant genes and pathways out of raw experimental data. The library provides a quality assessment of the signal intensities, as well as a broad range of options for data normalization, different statistical tests for the identification of significant siRNAs, and a significance analysis of the biological processes involving corresponding genes. The results of the analysis are presented as a set of HTML pages. Additionally, all values and plots are available as either text files or pdf and png files.

Availability: http://bioconductor.org/

Contact: RNAither@gmx.de

Journal Article.  1683 words. 

Subjects: Bioinformatics and Computational Biology

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