Journal Article

Genome-wide maps of mono- and di-nucleosomes of <i>Aspergillus fumigatus</i>

Hiromi Nishida, Takayuki Motoyama, Shogo Yamamoto, Hiroyuki Aburatani and Hiroyuki Osada

in Bioinformatics

Volume 25, issue 18, pages 2295-2297
Published in print September 2009 | ISSN: 1367-4803
Published online July 2009 | e-ISSN: 1460-2059 | DOI: http://dx.doi.org/10.1093/bioinformatics/btp413
Genome-wide maps of mono- and di-nucleosomes of Aspergillus fumigatus

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We identified 5 975 567 mono- and 6 995 122 di-nucleosome positions of the fungus Aspergillus fumigatus, which was detected at high resolution based on the DNA sequence data obtained from both mono- and di-nucleosomal DNA fragments. We show that the distribution of lengths of the mono-nucleosomal DNA fragments has two peaks at 135 and 150 nt, whereas the distribution of di-nucleosomal DNA fragment lengths has a single peak at 285 nt. Although the gene bodies of the active and inactive genes and the inactive gene promoters had the two peaks of the mono-nucleosomal DNA fragment lengths, the active gene promoter lost the longer peak at 150 nt. Our findings strongly suggest that the nucleosomes protecting longer DNA fragments against MNase at the promoters, thereby inhibiting high gene expression.

Contact: hnishida@iu.a.u-tokyo.ac.jp

Supplementary information: Supplementary data are available at Bioinformatics online.

Journal Article.  1415 words.  Illustrated.

Subjects: Bioinformatics and Computational Biology

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