Journal Article

Validation of double digest selective label database for sequenced prokaryotic genomes

Valery Terletskiy, Valentina Tyshchenko, Ilargi Martinez-Ballesteros, Javier Garaizar and Joseba Bikandi

in Bioinformatics

Volume 26, issue 3, pages 417-418
Published in print February 2010 | ISSN: 1367-4803
Published online December 2009 | e-ISSN: 1460-2059 | DOI: http://dx.doi.org/10.1093/bioinformatics/btp675
Validation of double digest selective label database for sequenced prokaryotic genomes

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Summary: A database for simulation of double digest selective label (DDSL) typing technique has been created and validated against a sequenced strain (Salmonella enterica serovar Typhimurium strain LT2). In silico bands were in agreement with experimental, and the technique was able to discriminate among strains belonging to the same species. When compared with other strain discrimination techniques, DDSL showed a higher discriminatory power. The database contains precomputed data which may be searched to retrieve experimental conditions for typing all up-to-dated sequenced prokaryotic microorganisms.

Availability: This is a new resource for molecular biology freely available on the Internet at http://insilico.ehu.es/DDSL

Contact: joseba.bikandi@ehu.es

Journal Article.  1250 words.  Illustrated.

Subjects: Bioinformatics and Computational Biology

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