Journal Article

NEMO: a tool for analyzing gene and chromosome territory distributions from 3D-FISH experiments

E. Iannuccelli, F. Mompart, J. Gellin, Y. Lahbib-Mansais, M. Yerle and T. Boudier

in Bioinformatics

Volume 26, issue 5, pages 696-697
Published in print March 2010 | ISSN: 1367-4803
Published online January 2010 | e-ISSN: 1460-2059 | DOI:
NEMO: a tool for analyzing gene and chromosome territory distributions from 3D-FISH experiments

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Summary: Three-dimensional fluorescence in situ hybridization (3D-FISH) is used to study the organization and the positioning of chromosomes or specific sequences such as genes or RNA in cell nuclei. Many different programs (commercial or free) allow image analysis for 3D-FISH experiments. One of the more efficient open-source programs for automatically processing 3D-FISH microscopy images is Smart 3D-FISH, an ImageJ plug-in designed to automatically analyze distances between genes. One of the drawbacks of Smart 3D-FISH is that it has a rather basic user interface and produces its results in various text and image files thus making the data post-processing step time consuming. We developed a new Smart 3D-FISH graphical user interface, NEMO, which provides all information in the same place so that results can be checked and validated efficiently. NEMO gives users the ability to drive their experiments analysis in either automatic, semi-automatic or manual detection mode. We also tuned Smart 3D-FISH to better analyze chromosome territories.

Availability: NEMO is a stand-alone Java application available for Windows and Linux platforms. The program is distributed under the creative commons licence and can be freely downloaded from


Journal Article.  1129 words.  Illustrated.

Subjects: Bioinformatics and Computational Biology

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