Journal Article

Filtering error from SOLiD Output

Ariella Sasson and Todd P. Michael

in Bioinformatics

Volume 26, issue 6, pages 849-850
Published in print March 2010 | ISSN: 1367-4803
Published online March 2010 | e-ISSN: 1460-2059 | DOI: https://dx.doi.org/10.1093/bioinformatics/btq045
Filtering error from SOLiD Output

More Like This

Show all results sharing this subject:

  • Bioinformatics and Computational Biology

GO

Show Summary Details

Preview

Summary: Here, we report the development of a filtering framework designed for efficient identification of both polyclonal and independent errors within SOLiD sequence data. The filtering utilizes the quality values reported by SOLiD's primary analysis for the identification of the two different types of errors. The filtering framework facilitates the passage of high-quality data into a variety of functional genomics applications, including de novo assemblers and sequence matching programs for SNP calling, improving the output quality and reducing resources necessary for analysis.

Availability: This error analysis framework is written in Perl and runs on Mac OS and Linux/Unix systems. The filter, documentation and sample Excel files for quality analysis are available at http://hts.rutgers.edu/filter and are distributed as Open Source software under the GPLv3.0.

Contact: tmichael@waksman.rutgers.edu

Supplementary information: Supplementary data is available at Bioinformatics online.

Journal Article.  1469 words.  Illustrated.

Subjects: Bioinformatics and Computational Biology

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.