Journal Article

Identification of protein complexes from co-immunoprecipitation data

Guy Geva and Roded Sharan

in Bioinformatics

Volume 27, issue 1, pages 111-117
Published in print January 2011 | ISSN: 1367-4803
Published online November 2010 | e-ISSN: 1460-2059 | DOI: http://dx.doi.org/10.1093/bioinformatics/btq652

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Motivation: Advanced technologies are producing large-scale protein–protein interaction data at an ever increasing pace. A fundamental challenge in analyzing these data is the inference of protein machineries. Previous methods for detecting protein complexes have been mainly based on analyzing binary protein–protein interaction data, ignoring the more involved co-complex relations obtained from co-immunoprecipitation experiments.

Results: Here, we devise a novel framework for protein complex detection from co-immunoprecipitation data. The framework aims at identifying sets of preys that significantly co-associate with the same set of baits. In application to an array of datasets from yeast, our method identifies thousands of protein complexes. Comparing these complexes to manually curated ones, we show that our method attains very high specificity and sensitivity levels (∼ 80%), outperforming current approaches for protein complex inference.

Availability: Supplementary information and the program are available at http://www.cs.tau.ac.il/~roded/CODEC/main.html.

Contact: roded@post.tau.ac.il

Supplementary information: Supplementary data are available at Bioinformatics online.

Journal Article.  5461 words.  Illustrated.

Subjects: Bioinformatics and Computational Biology

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