Journal Article

Efficient myelin repair in the macaque spinal cord by autologous grafts of Schwann cells

Corinne Bachelin, François Lachapelle, Christelle Girard, Pierre Moissonnier, Che Serguera-Lagache, Jacques Mallet, Denys Fontaine, Alexandre Chojnowski, Eric Le Guern, Brahim Nait-Oumesmar and Anne Baron-Van Evercooren

in Brain

Published on behalf of The Guarantors of Brain

Volume 128, issue 3, pages 540-549
Published in print March 2005 | ISSN: 0006-8950
Published online February 2005 | e-ISSN: 1460-2156 | DOI: http://dx.doi.org/10.1093/brain/awh406
Efficient myelin repair in the macaque spinal cord by autologous grafts of Schwann cells

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Experimental transplantation in rodent models of CNS demyelination has led to the idea that Schwann cells may be candidates for cell therapy in human myelin diseases. Here we investigated the ability of Schwann cells autografts to generate myelin in the demyelinated monkey spinal cord. We report that monkey Schwann cells derived from adult peripheral nerve biopsies retain, after growth factor expansion and transduction with a lentiviral vector encoding green fluorescent protein, the ability to differentiate in vitro into promyelinating cells. When transplanted in the demyelinated nude mouse spinal cord, they promoted functional and anatomical repair of the lesions (n = 12). Furthermore, we obtained evidence by immunohistochemistry (n = 2) and electron microscopy (n = 4) that autologous transplantation of expanded monkey Schwann cells in acute lesions of the monkey spinal cord results in the repair of large areas of demyelination; up to 55% of the axons were remyelinated by donor Schwann cells, the remaining ones being remyelinated by oligodendrocytes. Autologous grafts of Schwann cells may thus be of therapeutic value for myelin repair in the adult CNS.

Keywords: Schwann cell; primate; CNS; remyelination; GalC = galactocerebroside; GFP = green fluorescent protein; hrNDFβ = human recombinant neu differentiation factor β; IGF I = insulin-like growth factor 1; P0 = protein 0; PGK = phosphoglycerate kinase; SC = Schwann cells

Journal Article.  6113 words.  Illustrated.

Subjects: Neurology ; Neuroscience

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