Journal Article

<i>In vivo</i> measurement of axon diameter distribution in the corpus callosum of rat brain

Daniel Barazany, Peter J. Basser and Yaniv Assaf

in Brain

Published on behalf of The Guarantors of Brain

Volume 132, issue 5, pages 1210-1220
Published in print May 2009 | ISSN: 0006-8950
Published online April 2009 | e-ISSN: 1460-2156 | DOI:
In vivo measurement of axon diameter distribution in the corpus callosum of rat brain

Show Summary Details


Here, we present the first in vivo non-invasive measurement of the axon diameter distribution in the rat corpus callosum. Previously, this measurement was only possible using invasive histological methods. The axon diameter, along with other physical properties, such as the intra-axonal resistance, membrane resistance and capacitance etc. helps determine many important functional properties of nerves, such as their conduction velocity. In this work, we provide a novel magnetic resonance imaging method called AxCaliber, which can resolve the distinct signatures of trapped water molecules diffusing within axons as well as water molecules diffusing freely within the extra-axonal space. Using a series of diffusion weighted magnetic resonance imaging brain scans, we can reliably infer both the distribution of axon diameters and the volume fraction of these axons within each white matter voxel. We were able to verify the known microstructural variation along the corpus callosum of the rat from the anterior (genu) to posterior (splenium) regions. AxCaliber yields a narrow distribution centered ∼1 μm in the genu and splenium and much broader distributions centered ∼3 μm in the body of the corpus callosum. The axon diameter distribution found by AxCaliber is generally broader than those usually obtained by histology. One factor contributing to this difference is the significant tissue shrinkage that results from histological preparation. To that end, AxCaliber might provide a better estimate of the in vivo morphology of white matter. Being a magnetic resonance imaging based methodology, AxCaliber has the potential to be used in human scanners for morphological studies of white matter in normal and abnormal development, and white matter related diseases.

Keywords: MRI; brain; corpus callosum; axon diameter distribution; diffusion

Journal Article.  6360 words.  Illustrated.

Subjects: Neurology ; Neuroscience

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.