Journal Article

Release of sulfur mustard-modified DNA bases by Escherichia coli 3-methyladenine DNA glycosylase II

Zdenka Matijasevic, Allen Stering, Tian-Qi Niu, Paula Austin-Ritchie and David B. Ludlum

in Carcinogenesis

Volume 17, issue 10, pages 2249-2252
Published in print October 1996 | ISSN: 0143-3334
Published online October 1996 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/17.10.2249
Release of sulfur mustard-modified DNA bases by Escherichia coli
                    3-methyladenine DNA glycosylase II

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The toxic effects of sulfur mustard have been attributed to DNA modification with the formation of 7-hydroxyethyl-thioethyl guanine, 3-hydroxyethylthioethyl adenine and the cross-link, di-(2-guanin-7-yl-ethyl)sulfide. To investigate the action of bacterial 3-methyladenine DNA glycosylase II (Gly II) on these adducts, calf thymus DNA was modified with [14C]sulfur mustard and used as a substrate for Gly II. Gly II releases both 3-hydroxyethylthioethyl adenine and 7-hydroxyethylthioethyl guanine from this substrate. In comparison with the activity of Gly II towards methylated DNA, 3-hydroxyethylthioethyl adenine is released somewhat more slowly than 3-methyladenine, while 7-hydroxyethylthioethyl guanine is released much more readily than 7-methylguanine. Glycosylase action may play a role in protecting cells from the toxic effects of sulfur mustard.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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