Journal Article

SHORT COMMUNICATION: Glutathione modulates the formation of 8-hydroxydeoxyguannosine in isolated DNA and mutagenicity in <i>Salmonella typhimurium</i> TA100 induced by mineral fibres

Peter J. Howden and StephenP. Faux

in Carcinogenesis

Volume 17, issue 10, pages 2275-2277
Published in print October 1996 | ISSN: 0143-3334
Published online October 1996 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/17.10.2275
SHORT COMMUNICATION: Glutathione modulates the formation of
                    8-hydroxydeoxyguannosine in isolated DNA and mutagenicity in Salmonella
                        typhimurium TA100 induced by mineral fibres

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Treatment of isolated DNA with crocidolite and man-made vitreous fibre-21 (MMVF-21) significantly increased the concentration of 8-hydroxydeoxyguanosine (8-OHdG) in isolated DNA above background. levels and co-treatment with glutathione (GSH) eliminated this effect. Crocidolite, MMVF-21 and chrysolitle fibres increased the number of revertants in Salmonella typhimurium TA100 and GSH-deficient strains, TA100/NG-54 and TA100/NG-57, over background levels. This increase was small in TA100 but was greater in the GSH-deficient strains. When these bacterial strains were further depleted of GSH by co-culture with buthuonine sulfoximine, all fibres tested caused a significant increase in the number of revertants over the parent strains, Pre-treatment with the GSH precursor N-acetyl-L-cysteine reduced the number of revertants to below that of the parent strain. Previous studies have shown a mechanistic role for iron-catalyzed production of oxygen radicals in the mutagenicity of fibres and this study suggests a protective role for GSH against such oxidative damage possibly by acting as a radical scavenger.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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