Journal Article

Light-dependent activation of 7,12-dimethylbenz[<i>a</i>]anthracene to a potent genotoxicant

Serena Cinelli, Anita Falezza, Paola Ciliutti, Maurizio F. Mariani and J.-Albert Vericat

in Carcinogenesis

Volume 17, issue 11, pages 2529-5233
Published in print November 1996 | ISSN: 0143-3334
Published online November 1996 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/17.11.2529
Light-dependent activation of
                    7,12-dimethylbenz[a]anthracene to a potent
                    genotoxicant

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7, 12-Dimethylbenz[a]anthracene (DMBA), which is widely used in mutagenesis and experimental carcinogenesis, is activated to a mutagen by white fluorescent light. A 40 min exposure to white fluorescent light of Salmonella typhimurium TA98 plates treated with DMBA, in the absence of exogenous metabolism, resulted in an ∼30-fold increase in the number of histidine revertants. This phenomenon also occurs, with lesser intensity, with other promutagens, such as benzo[a]pyrene or 2-acetylamino-fluorene, and in other Salmonella tester strains. Moreover, white fluorescent light is able to activate DMBA to a toxicant for Chinese hamster V79 cells in culture, resulting in very low cell survival. Under these conditions, white fluorescent light-activated DMBA was shown to cause chromosomal aberrations, but not gene mutations, as determined by resistance to thioguanine. This white fluorescent light-dependent activation of DMBA seems to be related to the formation of reactive species, as the addition of vitamin E results in a reduction in the number of histidine revertants induced by white fluorescent light in S.typhimurium TA98.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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