Journal Article

Isolation and characterization of a novel gene induced by 2, 3, 7, 8-tetrachlorodibenzo-<i>p</i>-dioxin in rat liver

Ornella Selmin, George W. Lucier, George C. Clark, Angelika M. Tritscher, John P.Vanden Heuvel, Jonathan A. Gastel, Nigel J. Walker, Thomas R-Sutter and Douglas A. Bell

in Carcinogenesis

Volume 17, issue 12, pages 2609-2615
Published in print December 1996 | ISSN: 0143-3334
Published online December 1996 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/17.12.2609
Isolation and characterization of a novel gene induced by 2, 3, 7,
                        8-tetrachlorodibenzo-p-dioxin in rat liver

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The differential display technique was used to identify genes whose expression was regulated by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD). Expression of a novel sequence was up-regulated in a dose-dependent fashion in liver of Sprague-Dawley male rats exposed to both chronic and acute treatment with TCDD, as measured by densito-metry of Northern blot analyses (P < 0.01). A rapid amplification of cDNA ends (RACE) procedure was used to isolate a 1.8 kb cDNA from a rat liver cDNA preparation. This cloned cDNA, called 25-Dx, was sequenced and found to encode a peptide of 223 amino acids. In control rats, the 25-Dx gene was expressed at high levels in lung and liver. A hydrophobic domain of 14 residues followed by a proline-rich domain, both located in the N-terminal region, showed 71% homology with the transmembrane domain of the precursor for the interleukin-6 receptor and a conserved consensus sequence found in the cytokine/growth factor/prolactin receptor superfamily respectively.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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