Journal Article

<i>Hprt</i> mutations in human T-lymphocytes reflect radioprotective effects of the aminothiol, WR-1065

L.Scott Clark, Richard J. Albertini and Janice A-Nicklas

in Carcinogenesis

Volume 17, issue 12, pages 2647-2653
Published in print December 1996 | ISSN: 0143-3334
Published online December 1996 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/17.12.2647
Hprt mutations in human T-lymphocytes reflect
                    radioprotective effects of the aminothiol, WR-1065

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Aminothiols such as WR-2721 and its active free thiol WR-1065 have previously been shown to reduce mutations resulting from ionizing radiation in exponentially growing cells. In this study, non-dividing human Go T-lymphocytes were exposed to the aminothiol radioprotective agent, WR-1065, 30 min before or 3 h after external beam γ-irradiation and subsequently assessed for survival and mutation induction at the hprt locus. Cytotoxicity due to γ-irradiation was reduced only when the WR-1065 was present during irradiation. The frequency of hprt mutations, however, was reduced regardless of time of administration, although the reduction was statistically significant only when WR-1065 was added 30 min before irradiation (P < 0.01). This is the first study to demonstrate the protective effects of WR-1065 against radiation-induced mutation in a reporter gene using a human non-cycling cell. Hprt mutations arising in vivo in these cells may be useful for monitoring the radioprotective effect of aminothiols in human populations.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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