Journal Article

Mutations and oxidative DNA damage in phage M13mp2 exposed to <i>N</i>-nitrosomorpholine plus near-ultraviolet light

Miho Fujiwara, Yuko Honda, Hideo Inoue, Hikoya Hayatsu and Sakae Arimoto

in Carcinogenesis

Volume 17, issue 2, pages 213-218
Published in print February 1996 | ISSN: 0143-3334
Published online February 1996 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/17.2.213
Mutations and oxidative DNA damage in phage M13mp2 exposed to N-nitrosomorpholine plus near-ultraviolet light

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Previously we reported that a direct-acting mutagen can be formed from N-nitrosomorpholine (NMOR) on exposure to near-ultraviolet light (UVA, 320-400 nm). We have now studied the spectrum of mutations caused by NMOR plus UVA. M13mp2 phages suspended in a sodium phosphate buffer were treated with NMOR under UVA irradiation and Escherichia coli NR9099 was then infected with the phage. Mutations induced in the phage DNA lacZα region were analyzed. The majority (∼50%) of the induced sequence changes were G to T transversions. This suggested that modifications in guanine residues were responsible for these transversions. We explored the formation of 7,8-dihydro-8-oxodeoxyguanosine (8-oxodG) in the DNA. When the phage were treated with NMOR plus UVA, 8-oxodG/dG in DNAincreased up to 12-fold over the value in untreated control. When a mutM-deficient mutant of E.coti CSH50 was used as the host, the mutation level was higher than that observed with CSH50. We conclude that 8-oxodG may be involved in mutations induced by NMOR plus UVA.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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