Journal Article

Tobacco smoke-associated <i>N</i>7-alkylguanine in DNA of larynx tissue and leucocytes

K. Szyfter, K. Hemminki, W. Szyfter, Z. Szmeja, J. Banaszewski and M. Pabiszczak

in Carcinogenesis

Volume 17, issue 3, pages 501-506
Published in print March 1996 | ISSN: 0143-3334
e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/17.3.501
Tobacco smoke-associated N7-alkylguanine in DNA of larynx tissue and leucocytes

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The presence of N7-alkylguanine adducts in DNA was analysed in a group of 46 patients with larynx tumours. All patients were subjected to laryngectomy and the tissues accessible for analysis by 32P-post-labelling assay were larynx tumour, larynx non-tumour and peripheral blood leucocytes. N7-Alkylguanine adducts were detected in all the studied DNA samples. The average level of N7-alkylguanines was 26.2/107 nucleotides in tumour cells, 22.7/107 in non-tumour cells and 13.1/107 in blood leucocytes. There was a significantly higher level of N7-alkylguanines in the larynx tissues in males than in females. The effect of tobacco smoking on DNA adduct levels was shown by an increase in the average levels of N7-alkylguanines in the subject groups classified according to their smoking habits. A moderate age-related increase in levels of N7-alkylguanine was demonstrated in larynx tumour tissue. The levels of N7-alkylguanine adducts in larynx cells were compared with that of aromatic DNA adducts. Pearson correlation coefficients (0.28 for tumour tissue and 0.30 for non-tumour tissue) indicate independent formation and removal of N7-alkylguanine and aromatic DNA adducts resulting from tobacco smoke exposure.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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