Journal Article

Presence of <i>N</i><sup>2</sup>-(deoxyguanosin-8-yl)-2-amino-3,8-dimethylimidazo[4,5-<i>f</i>]quinoxaline (dG-C8-MeIQx) in human tissues

Yukari Totsuka, Kazuo Fukutome, Mami Takahashi, Satoru Takahashi, Akihiro Tada, Takashi Sugimura and Keiji Wakabayashi

in Carcinogenesis

Volume 17, issue 5, pages 1029-1034
Published in print May 1996 | ISSN: 0143-3334
e-ISSN: 1460-2180 | DOI: https://dx.doi.org/10.1093/carcin/17.5.1029
Presence of N2-(deoxyguanosin-8-yl)-2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (dG-C8-MeIQx) in human tissues

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One of the mutagenic and carcinogenic heterocyclic amines (HCAs), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MelQx), is present in cooked foods and we are chronically exposed to this compound in our daily life. To study the role of HCAs in human carcinogenesis, we analyzed MelQx-DNA adducts in 38 DNA samples obtained from surgical and autopsy specimens by the 32P-postlabeling method under adduct-intensification conditions with the modification of additional digestion with nuclease P1 and phosphodiesterase I after 32P-labeling at 5′ -hydroxyl termini. This modified 32P-postlabeling method can detect N2-(deoxyguanosin-8-yl)-2-amino-3,8-dimethylimidazo[4, 5-f]quinoxaline 5′-monophos-phate (5′-pdG-C8-MeIQx) at levels down to 1/1010 nucleo-tides. The DNA samples from colon and rectum surgical specimens and a kidney taken at autopsy were found to contain an adduct spot corresponding to that of standard 5′-pdG-C8-MeIQx on TLC at levels of 14, 18 and 1.8 per 1010 nucleotides, respectively. Each adduct spot was extracted from TLC and identified to be 5′-pdG-C8-MeIQx by HPLC. Thus, MelQx-DNA adducts actually exist in human tissues and this adduct formation may be involved in human cancer development.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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