Journal Article

Characterization of an N<sup>6</sup>-oxopropenyl-′-deoxyadenosine adduct in malondialdehyde-modified DNA using liquid chromatography/electrospry ionization tandem mass spectrometry

Ajai K. Chaudhary, G.Ramachandra Reddy, Ian A. Blair and Lawrence J. Marnett

in Carcinogenesis

Volume 17, issue 5, pages 1167-1170
Published in print May 1996 | ISSN: 0143-3334
e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/17.5.1167
Characterization of an N6-oxopropenyl-′-deoxyadenosine adduct in malondialdehyde-modified DNA using liquid chromatography/electrospry ionization tandem mass spectrometry

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Malondialdehyde (MDA), a product of lipid peroxidation, causes mutations in bacterial and mammalian cells and cancer in rats. MDA reacts with deoxynucleosides in vitro and the monomeric adduct of MDA with deoxyguanosine (M1G-dR) is the major adduct M1G-dR has been detected in rat and human liver. Random mutagenesis studies with MDA-modified DNA and recent 32P-postlabeling studies indicate that in addition to M1G-dR, adducts to deoxyadenosine may also be formed. We have utilized liquid chromatography coupled with electrospray ionization tandem mass spectrometry to characterize an N6-oxopropenyl-2′-deoxyadenosine adduct (M1A-dR) in calfthymus DNA modified with MDA.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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