Journal Article

Kinds of mutations induced by 3-chloro-4-(dichloromethyl)-5-hydroxy-2(<i>5H</i>)-furanone (MX) in the <i>hprt</i> gene of Chinese hamster ovary cells

Juha M.T. Hyttinen, Sanna Myöhänen and Kristian Jansson

in Carcinogenesis

Volume 17, issue 5, pages 1179-1181
Published in print May 1996 | ISSN: 0143-3334
e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/17.5.1179
Kinds of mutations induced by 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) in the hprt gene of Chinese hamster ovary cells

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The kinds of mutations induced by 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) in the protein coding region of the hprt gene of Chinese hamster ovary (CHO) cells were determined by direct sequencing of polymerase chain reaction (PCR)-amplified cDNA. Primary mutations were found in 15 of 19 of the mutants: 11 were G:C⇒T:A transversions, two were A:T⇒T:A transversions and two were deletions of single G:C base pairs (-1 frameshifts). The remaining four mutants had large alterations in the cDNA that were explained by mRNA splicing errors. A group of control mutants had more diverse hprt cDNA alterations than MX-induced mutants. Transversions yielding an A:T base pair were the predominant type of MX-induced mutations, in agreement with previous findings in bacteria. This specificity may be explained by the ‘A rule’, that DNA polymerases preferentially insert, adenine nucleotides opposite non-instructional lesions.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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