Journal Article

Assay of different photoproducts after UVA, B and C irradiation of DNA and human skin explants

Vladimir J. Bykov and Kari Hemminki

in Carcinogenesis

Volume 17, issue 9, pages 1949-1955
Published in print September 1996 | ISSN: 0143-3334
Published online September 1996 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/17.9.1949
Assay of different photoproducts after UVA, B and C irradiation of
                    DNA and human skin explants

More Like This

Show all results sharing this subject:

  • Clinical Cytogenetics and Molecular Genetics

GO

Show Summary Details

Preview

A 32P-HPLC method was applied to study the induction of UVB- and UVC-induced DNA lesions (cyclobutane dimers, 6-4 photoproducts and Dewar isomers) in human skin explants. The employed technique was sensitive enough to detect the lesions at a dose of 10 J/m2 UVB. Comparison of photoadduct formation under UVC and UVB indicated the importance of photosensitization pathways in DNA damage. Dewar isomers were detected only at a high dose of UVB. The compounds were identified by their photochemical reactivity and by spiking with prepared standards. Treatment with nuclease P1 was used to identify the 5'-terminal nucleotide. UVA caused no detectable adducts.

Journal Article.  0 words. 

Subjects: Clinical Cytogenetics and Molecular Genetics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.