Journal Article

Evaluation of DNA repair inhibition by antitumor or antibiotic drugs using a chemiluminescence microplate assay.

J M Barret, B Salles, C Provot and B T Hill

in Carcinogenesis

Volume 18, issue 12, pages 2441-2445
Published in print December 1997 | ISSN: 0143-3334
Published online December 1997 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/18.12.2441
Evaluation of DNA repair inhibition by antitumor or antibiotic drugs using a chemiluminescence microplate assay.

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A recently derived in vitro chemiluminescence assay (Salles et al. [1995] Anal. Biochem., 232, 37-42) has been used to investigate the effects of a panel of twenty-two anticancer drugs and certain antibiotics on the excision repair activity of cell-free extracts from the human cell line, HeLa. This methodology, termed the 3D (Damaged DNA Detection) assay, based on the in vitro excision repair assay previously developed (Wood et al. [1988] Cell, 53, 97-106) has provided data indicating definite in vitro inhibition of DNA repair by actinomycin D, aphidicolin, doxorubicin, distamycin A and mithramycin A. This assay therefore offers the potential for identifying agents with the ability to inhibit DNA repair.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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