Journal Article

Inhibition by 1'-acetoxychavicol acetate of lipopolysaccharide- and interferon-gamma-induced nitric oxide production through suppression of inducible nitric oxide synthase gene expression in RAW264 cells.

T Ohata, K Fukuda, A Murakami, H Ohigashi, T Sugimura and K Wakabayashi

in Carcinogenesis

Volume 19, issue 6, pages 1007-1012
Published in print June 1998 | ISSN: 0143-3334
Published online June 1998 | e-ISSN: 1460-2180 | DOI: https://dx.doi.org/10.1093/carcin/19.6.1007
Inhibition by 1'-acetoxychavicol acetate of lipopolysaccharide- and interferon-gamma-induced nitric oxide production through suppression of inducible nitric oxide synthase gene expression in RAW264 cells.

More Like This

Show all results sharing this subject:

  • Clinical Cytogenetics and Molecular Genetics

GO

Show Summary Details

Preview

Although nitric oxide (NO) is an important biological mediator, excessive production in inflammation is thought to be a causative factor of cellular injury and cancer in the long term. In the present study the effects of 1'-acetoxychavicol acetate (ACA), which has anticarcinogenic properties, on NO production in murine macrophage cell line RAW264 cells stimulated with lipopolysaccharide or interferon-gamma were examined. ACA suppressed NO production dose dependently with an IC50 of 160 ng/ml (680 nM). The decrease in NO production was shown to parallel reduced expression of iNOS mRNA and protein. The influence of ACA on transcription factors, such as NF-kappaB, AP-1 and Stat1, which are involved in expression of the iNOS gene was assessed. ACA was found to suppress degradation of IkappaB, an NF-kappaB inhibitory factor, and consequently inhibit NF-kappaB activation. Activation of AP-1 and Stat1 was also blocked by ACA treatment. Thus we demonstrate that ACA exerts potent inhibitory effects on NO production, apparently mediated by modulation of activation of several transcription factors. This could contribute to the anticarcinogenic properties of ACA.

Journal Article.  0 words. 

Subjects: Clinical Cytogenetics and Molecular Genetics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.