Journal Article

X-radiation induces 8-hydroxy-2'-deoxyguanosine formation in vivo in rat mammary gland DNA.

A D Haegele, P Wolfe and H J Thompson

in Carcinogenesis

Volume 19, issue 7, pages 1319-1321
Published in print July 1998 | ISSN: 0143-3334
Published online July 1998 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/19.7.1319
X-radiation induces 8-hydroxy-2'-deoxyguanosine formation in vivo in rat mammary gland DNA.

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Ionizing radiation is a carcinogen that induces oxidative DNA damage. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a relatively abundant, mutagenic lesion that is widely regarded as a reliable index of oxidative DNA damage. The purpose of this study was to examine the effects of X-radiation on levels of 8-OHdG in the context of an experimental model for breast cancer in which chronic radiation exposure has been shown to be carcinogenic in Sprague-Dawley rats. A secondary objective of this study was to determine if the use of phenol during DNA isolation affected the concentration of 8-OHdG subsequently measured. Our results indicate that a profoundly carcinogenic dose of radiation induced a small but significant increase in 8-OHdG concentration in mammary gland DNA, and that the use of a phenol-based versus a salt-based method of DNA isolation had no significant impact on the levels of 8-OHdG detected in either control or irradiated tissue.

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Subjects: Clinical Cytogenetics and Molecular Genetics

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