Journal Article

A hypothesis for what conformation of the major adduct of (+)-<i>anti</i>-B[<i>a</i>]PDE (<i>N</i><sup>2</sup>-dG) causes G→T versus G→A mutations based upon a correlation between mutagenesis and molecular modeling results

Richard E. Kozack, Rajiv Shukla and Edward L. Loechler

in Carcinogenesis

Volume 20, issue 1, pages 95-102
Published in print January 1999 | ISSN: 0143-3334
Published online January 1999 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/20.1.95
A hypothesis for what conformation of the major adduct of (+)-anti-B[a]PDE (N2-dG) causes G→T versus G→A mutations based upon a correlation between mutagenesis and molecular modeling results

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Molecular modeling (simulated annealing) was used to study the conformations in dsDNA of [+ta]-B[a]P–N2-dG (R.E.Kozack and E.L.Loechler, accompanying paper), which is the major benzo[a]pyrene (B[a]P) adduct. Sixteen classes of conformations were identified, and are analyzed herein vis-a-vis the two most prominent B[a]P mutations, G→T and G→A base substitutions. Eight conformations seem more relevant to frameshift mutagenesis, so they are excluded, leaving eight conformations as follows. Two conformations (BPmi5 and BPmi3) retain Watson–Crick G:C base pairing having the B[a]P moiety of the adduct in the minor groove. Two conformations (BPma5 and BPma3) have the Hoogsteen orientation with B[a]P in the major groove. Four conformations are base displaced and have B[a]P stacked in the helix with the dG moiety of the adduct displaced into either the major groove (Gma5 and Gma3) or the minor groove (Gmi5 and Gmi3). Three of these eight conformations (BPma5, BPma3 and Gma3) are universally high in energy. The two conformations that retain G:C base pairing potential (BPmi5 and BPmi3) are likely to be non-mutagenic. Of the three remaining conformations, Gmi5 can be relatively low in energy, but is distorted. A correlation exists between the calculated energies for the remaining two base displaced conformations and mutagenesis for [+ta]-B[a]P–N2-dG, leading to the hypothesis that Gma5 is responsible for G→T mutations and Gmi3 is responsible for G→A mutations. Gma5 and Gmi3 resemble each other, except that dG is in the major and minor grooves, respectively. An incipient rationale for this hypothesis is discussed: DNA polymerase might be triggered to follow a different mutagenic pathway depending upon whether a non-informational lesion has bulk protruding into the major or minor groove. A pathway for interconversion between these eight conformations is also proposed and its implications are discussed; e.g. four steps are required to interconvert between Gma5 and Gmi3.

Keywords: (+)-anti-B[a]PDE, (+)-r-7,t-8-dihydroxy-t-9,10-epoxy-7,8,9,10,-tetrahydrobenzo[a]pyrene (anti); B[a]P, benzo[a]pyrene; [+ta]-B[a]P–N2-dG, the major adduct of (+)-anti-B[a]PDE formed by trans addition of N2-dG to (+)-anti-B[a]PDE; ds, double-stranded; ss, single-stranded.

Journal Article.  5089 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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