Journal Article

Effects of environmental estrogens on tumor necrosis factor α-mediated apoptosis in MCF-7 cells

Matthew E Burow, Yan Tang, Bridgette M. Collins-Burow1, Stanislaw Krajewski, John C. Reed, John A. McLachlan and Barbara S Beckman

in Carcinogenesis

Volume 20, issue 11, pages 2057-2061
Published in print November 1999 | ISSN: 0143-3334
Published online November 1999 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/20.11.2057
Effects of environmental estrogens on tumor necrosis factor α-mediated apoptosis in MCF-7 cells

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Environmental estrogens represent a class of compounds which have been shown to mimic the effects or activity of the naturally occurring ovarian hormone 17β-estradiol. Given the role of 17β-estradiol in cell survival in a number of systems, we wished to determine if environmental estrogens protect MCF-7 cells from apoptosis. Here we demonstrate that the organochlorine pesticides o, p′ DDT and alachlor, like 17β-estradiol, have the ability to suppress tumor necrosis factor α (TNF)-induced apoptosis in estrogen receptor (ER)-positive MCF-7 breast carcinoma cells. These compounds, however, did not affect TNF-induced apoptosis of the ER-negative MDA-MB-231 cell line. The ability of these compounds to suppress apoptosis in MCF-7 cells was correlated with an ER-dependent increase in Bcl-2 expression. Taken together these results demonstrate that estrogenic organochlorine pesticides like o, p′ DDT and alachlor may partially mimic the primary endogenous estrogen, 17β-estradiol, and function to suppress apoptosis in ER-responsive cells.

Keywords: alachlor, 2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide; o,p′ DDT, 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) ethane; DMEM, Dulbecco's modified Eagle's medium; endosulfan II (3α,5aβ,6β,9β,9aβ)-6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepin 3-oxide: ER, estrogen receptor; ERE, estrogen response element; FBS, fetal bovine serum; TNF, tumor necrosis factor α.

Journal Article.  3784 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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