Journal Article

Prevention of UVB-induced immunosuppression in mice by the green tea polyphenol (–)-epigallocatechin-3-gallate may be associated with alterations in IL-10 and IL-12 production

Santosh K. Katiyar, Anjana Challa, Thomas S. McCormick, Kevin D. Cooper and Hasan Mukhtar

in Carcinogenesis

Volume 20, issue 11, pages 2117-2124
Published in print November 1999 | ISSN: 0143-3334
Published online November 1999 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/20.11.2117
Prevention of UVB-induced immunosuppression in mice by the green tea polyphenol (–)-epigallocatechin-3-gallate may be associated with alterations in IL-10 and IL-12 production

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UV exposure of the skin, particularly UVB (290–320 nm), causes adverse biological effects, including alterations in cutaneous immune cells, photoaging and photocarcinogenesis. Several studies have shown that polyphenolic compounds isolated from green tea afford protection against UVB-induced inflammatory responses and photocarcinogenesis in murine models. In this study we show that topical application of (–)-epigallocatechin-3-gallate (EGCG) (3 mg/mouse), a major polyphenolic component of green tea, before a single low dose UVB exposure (72 mJ/cm2) to C3H/HeN mice prevented UVB-induced inhibition of the contact hypersensitivity response and tolerance induction to the contact sensitizer 2,4-dinitrofluorobenzene. Topical application of EGCG before UVB exposure reduced the number of CD11b+ monocytes/macrophages and neutrophils infiltrating into skin inflammatory lesions, which are considered to be responsible for creating the UV-induced immunosuppressive state. In addition, application of EGCG before UVB exposure decreased UVB-induced production of the immunomodulatory cytokine interleukin (IL)-10 in skin as well as in draining lymph nodes (DLN), whereas production of IL-12, which is considered to be a mediator and adjuvant for induction of contact sensitivity, was found to be markedly increased in DLN when compared with UVB alone-exposed mice. Taken together, our data demonstrate that EGCG protects against UVB-induced immunosuppression and tolerance induction by: (i) blocking UVB-induced infiltration of CD11b+ cells into the skin; (ii) reducing IL-10 production in skin as well as in DLN; (iii) markedly increasing IL-12 production in DLN. Protection against UVB-induced immunosuppression by EGCG may be associated with protection against UVB-induced photocarcinogenesis.

Keywords: APC, antigen-presenting cell; CHS, contact hypersensitivity; DAB, diaminobenzidine; DLN, draining lymph node; DNFB, 2,4-dinitrofluorobenzene; EGCG, (–)-epigallocatechin-3-gallate; FBS, fetal bovine serum; IL, interleukin.

Journal Article.  6615 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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