Journal Article

Safrole-like DNA adducts in oral tissue from oral cancer patients with a betel quid chewing history

Chiu-Lan Chen, Chin-Wen Chi, Kuo-Wei Chang and Tsung-Yun Liu

in Carcinogenesis

Volume 20, issue 12, pages 2331-2334
Published in print December 1999 | ISSN: 0143-3334
Published online December 1999 | e-ISSN: 1460-2180 | DOI:
Safrole-like DNA adducts in oral tissue from oral cancer patients with a betel quid chewing history

More Like This

Show all results sharing this subject:

  • Clinical Cytogenetics and Molecular Genetics


Show Summary Details


Betel quid (BQ) chewing has been associated with an increased risk of oral squamous cell carcinoma (OSCC) and oral submucous fibrosis (OSF). Piper betle inflorescence, which contains 15 mg/g safrole, is a unique ingredient of BQ in Taiwan. Chewing such prepared BQ may contribute to safrole exposure in human beings (420 μM safrole in saliva). Safrole is a known rodent hepatocarcinogen, yet its carcinogenicity in human beings is largely undetermined. In this study, using a 32P-post-labeling method, we have found a high frequency of safrole-like DNA adducts in BQ-associated OSCC (77%, 23/30) and non-cancerous matched tissue (NCMT) (97%, 29/30). This was in contrast to the absence (< 1/109 nucleotides) of such adducts in all of non-BQ-associated OSCC and their paired NCMT (P < 0.001). Six of seven OSF also exhibited the same safrole-like DNA adduct. The DNA adduct levels in OSF and NCMT were significantly higher than in OSCC (P < 0.05). Using co-chromatography and rechromatography techniques, we further demonstrated that these adducts were identical to synthetic safrole–dGMP adducts as well as DNA adducts from 1′-hydroxysafrole-treated HepG2 cells. These results suggest that safrole forms stable safrole–DNA adducts in human oral tissue following BQ chewing, which may contribute to oral carcinogenesis.

Keywords: BQ, betel quid; dAMP, 2′-deoxyadenosine 3′-monophosphate; dGMP, 2′-deoxyguanosine 3′-monophosphate; NCMT, non-cancerous matched tissue; OSCC, oral squamous cell carcinoma; OSF, oral submucous fibrosis; PAPS, 3′-phosphoadenosine 5′-phosphosulfate.

Journal Article.  3173 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.