Journal Article

Identification of the major tamoxifen–DNA adducts in rat liver by mass spectroscopy

Heli Rajaniemi, Ilpo Rasanen, Pertti Koivisto, Kimmo Peltonen and Kari Hemminki

in Carcinogenesis

Volume 20, issue 2, pages 305-309
Published in print February 1999 | ISSN: 0143-3334
Published online February 1999 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/20.2.305
Identification of the major tamoxifen–DNA adducts in rat liver by mass spectroscopy

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We present here the first mass spectroscopic (MS) identification of the main tamoxifen-induced DNA adducts in rat liver. The two main adducts were isolated by high-performance liquid chromatography (HPLC) and identified by MS, MS–MS and ultraviolet spectroscopy. Adduct 1 was the N-desmethyltamoxifen–deoxyguanosine adduct in which the α-position of the metabolite N-desmethyltamoxifen is linked covalently to the amino group of deoxyguanosine. Adduct 2 was confirmed to be the trans isomer of α-(N2-deoxyguanosinyl)tamoxifen, as previously suggested by co-chromatography.

Keywords: dGMP, deoxyguanosine 5′-monophosphate; HPLC, high-performance liquid chromatography; MS, mass spectroscopy; NP1, nuclease P1.

Journal Article.  2939 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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