Journal Article

HPLC/fluorescence determination of <i>anti</i>-BPDE–DNA adducts in mononuclear white blood cells from PAH-exposed humans

Sofia Pavanello, Donata Favretto, Francesco Brugnone, Giuseppe Mastrangelo, Giorgio Dal Pra and Erminio Clonfero

in Carcinogenesis

Volume 20, issue 3, pages 431-435
Published in print March 1999 | ISSN: 0143-3334
Published online March 1999 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/20.3.431
HPLC/fluorescence determination of anti-BPDE–DNA adducts in mononuclear white blood cells from PAH-exposed humans

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The aim of this study was to compare (±)-r-7,t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (anti-BPDE)–DNA adduct levels in groups of humans subjected to various levels of polycyclic aromatic hydrocarbon (PAH) (benzo[a]pyrene) exposure. An HPLC/fluorescence method was applied to detect specifically anti-BPDE–DNA adducts in mononuclear white blood cells [lymphocyte plus monocyte fraction (LMF)] from humans exposed to PAHs. A total of 130 subjects comprised the sample population: 26 psoriatic patients (3 days after clinical coal tar treatment of the skin), 15 coke oven workers, 19 chimney sweeps, 36 aluminium anode plant workers and 34 non-occupationally PAH-exposed subjects (controls). PAH exposure was assessed in each group by means of the urinary excretion of 1-pyrenol (mean group levels: 1.2, 0.7, 0.3, 65.0 and 0.1 μmol/mol creatinine in coke oven workers, chimney sweeps, aluminium plant anode workers, psoriatic patients and non-occupationally PAH-exposed subjects, respectively). HPLC/fluorescence analysis of BPDE–DNA adducts showed that the percentage of subjects with adduct levels exceeding the 95 percentile control subject value (8.9 adducts/108 nucleotides) was significantly high in coke oven workers (46.7%) and chimney sweeps (21.0%) (χ2 test, P < 0.01 and P < 0.05, respectively) but not in aluminium plant workers (11.1%) and psoriatic patients (0%). The increase in BPDE–DNA adduct levels in LMF (Ln values) was significantly related to chronic inhalatory and high PAH exposure (linear multiple regression analysis, F = 6.37, P < 0.01; t = 4.2, P < 0.001). Skin acute (or short-term) and high PAH exposure, charcoal-grilled meat consumption and smoking habit did not seem to influence BPDE–DNA adduct formation in LMF.

Keywords: anti-BPDE, (±)-r-7,t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene; B[a]P, benzo[a]pyrene; B[a]P-tetrol I-1, r-7,c-10,t-8,t-9-tetrahydroxy-7,8,9,10-tetrahydro-benzo[a]pyrene; CT, coal tar; CYP, cytochrome P450; GST, glutathione S-transferase; LMF, lymphocyte plus monocyte fraction; PAH, polycyclic aromatic hydrocarbon; TLC, thin-layer chromatography; WBC, white blood cell.

Journal Article.  5154 words. 

Subjects: Clinical Cytogenetics and Molecular Genetics

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