Journal Article

<i>Helicobacter pylori</i> infection enhances glandular stomach carcinogenesis in Mongolian gerbils treated with chemical carcinogens

Nobuyuki Shimizu, Ken-ichi Inada, Hayao Nakanishi, Tetsuya Tsukamoto, Yuzuru Ikehara, Michio Kaminishi, Shu Kuramoto, Atsushi Sugiyama, Tsutomu Katsuyama and Masae Tatematsu

in Carcinogenesis

Volume 20, issue 4, pages 669-676
Published in print April 1999 | ISSN: 0143-3334
Published online April 1999 | e-ISSN: 1460-2180 | DOI: https://dx.doi.org/10.1093/carcin/20.4.669
Helicobacter pylori infection enhances glandular stomach carcinogenesis in Mongolian gerbils treated with chemical carcinogens

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Helicobacter pylori (Hp) is thought to be a stomach carcinogen from epidemiological findings. To determine the effects of infection with the bacteria on experimental carcinogenesis, a study of the glandular stomach of Mongolian gerbils (MGs) was performed. Male MGs were treated with N-methyl-N′-nitro-N-nitrosoguanidine followed by inoculation with Hp or infected with Hp followed by N-methyl-N′-nitro-N-nitrosoguanidine administration. Animals were killed at week 50, and their excised stomachs underwent microbiological and histopathological examinations. In addition, a serological investigation was performed. The incidences of adenocarcinomas were significantly higher in animals treated with 60 or 300 p.p.m. N-methyl-N′-nitro-N-nitrosoguanidine for 10 weeks followed by Hp inoculation or Hp followed by 20 p.p.m. N-methyl-N′-nitro-N-nitrosoguanidine for 30 weeks than in the respective controls. Moreover, tumour-bearing animals had higher titres of anti-Hp antibodies than tumour-free animals. Of interest was the finding that a dose of 100 p.p.m. N-methyl-N′-nitro-N-nitrosoguanidine given to infected gerbils eradicated the Hp in about half the animals, with a concomitant reduction in the promoting effect. No tumours were found in animals infected with Hp without N-methyl-N′-nitro-N-nitrosoguanidine or non-treated gerbils. Hp infection enhances glandular stomach carcinogenesis in MGs treated with N-methyl-N′-nitro-N-nitrosoguanidine. Animals with high titres of anti-Hp antibodies are at greatest risk of developing neoplasms.

Keywords: Hp, Helicobacter pylori; MG, Mongolian gerbil; MNU, N-methyl-N-nitrosourea; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; PBS, phosphate-buffered saline.

Journal Article.  6071 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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