Journal Article

Lack of effect of a 60 Hz magnetic field on biomarkers of tumor promotion in the skin of SENCAR mice

John DiGiovanni, Dennis A. Johnston, Tim Rupp, Lyle B. Sasser, Larry E. Anderson, James E. Morris, Douglas L. Miller, Robert Kavet and Earl F. Walborg

in Carcinogenesis

Volume 20, issue 4, pages 685-689
Published in print April 1999 | ISSN: 0143-3334
Published online April 1999 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/20.4.685
Lack of effect of a 60 Hz magnetic field on biomarkers of tumor promotion in the skin of SENCAR mice

More Like This

Show all results sharing this subject:

  • Clinical Cytogenetics and Molecular Genetics

GO

Show Summary Details

Preview

It has been proposed that extremely low frequency magnetic fields may enhance tumorigenesis through a co-promotional mechanism. This hypothesis has been further tested using the two-stage model of mouse skin carcinogenesis, i.e. 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced promotion of skin carcinogenesis in mice initiated by a single subcarcinogenic dose of 7,12-dimethylbenz-[a]anthracene. Experimentation utilized three different doses of TPA within its dose–response range (0.85, 1.70 or 3.40 nmol) and examined the following early biomarkers of tumor promotion after 1, 2 and 5 weeks of promotion: increases in epidermal thickness and the labeling index of epidermal cells, induction of epidermal ornithine decarboxylase activity and down-regulation of epidermal protein kinase C activity. Mice exposed to a 60 Hz magnetic field having a flux density of 2 mT for 6 h/day for 5 days/week were compared with mice exposed to an ambient magnetic field. Within the sensitivity limits of the biomarker methodology and the exposure parameters employed, no consistent, statistically significant effects indicative of promotion or co-promotion by the magnetic field were demonstrated.

Keywords: BrdUrd, 2-bromodeoxyuridine; DMBA, dimethylbenz[a]anthracene; ELF, extremely low frequency; ODC, ornithine decarboxylase; PKC, protein kinase C; TPA, 12-O-tetradecanoylphorbol-13-acetate.

Journal Article.  5124 words. 

Subjects: Clinical Cytogenetics and Molecular Genetics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.