Journal Article

Induction of oxidative stress and oxidative damage in rat glial cells by acrylonitrile

L.M. Kamendulis, J. Jiang, Y. Xu and J.E. Klaunig

in Carcinogenesis

Volume 20, issue 8, pages 1555-1560
Published in print August 1999 | ISSN: 0143-3334
Published online August 1999 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/20.8.1555
Induction of oxidative stress and oxidative damage in rat glial cells by acrylonitrile

More Like This

Show all results sharing this subject:

  • Clinical Cytogenetics and Molecular Genetics

GO

Show Summary Details

Preview

Chronic treatment of rats with acrylonitrile (ACN) resulted in a dose-related increase in glial cell tumors (astrocytomas). While the exact mechanism(s) for ACN-induced carcinogenicity remains unresolved, non-genotoxic and possibly tumor promotion modes of action appear to be involved in the induction of glial tumors. Recent studies have shown that ACN induced oxidative stress selectively in rat brain in a dose-responsive manner. The present study examined the ability of ACN to induce oxidative stress in a rat glial cell line, a target tissue, and in cultured rat hepatocytes, a non-target tissue of ACN carcinogenicity. Glial cells and hepatocytes were treated for 1, 4 and 24 h with sublethal concentrations of ACN. ACN induced an increase in oxidative DNA damage, as evidenced by increased production of 8-hydroxy-2′-deoxyguanosine (8-OH-dG) in glial cells but not in rat hepatocytes. Hydroxyl radical formation following ACN treatment was also selectively increased in glial cells. Following 1 and 4 h of ACN exposure, the levels of the non-enzymatic antioxidant glutathione, as well as the activities of the enzymatic antioxidants catalase and superoxide dismutase were significantly decreased in the rat glial cells. Lipid peroxidation and the activity of glutathione peroxidase were not affected by ACN treatment in rat glial cells. No changes in any of these biomarkers of oxidative stress were observed in hepatocytes treated with ACN. These data indicate that ACN selectively induced oxidative stress in rat glial cells.

Keywords: 2,3-DHBA, 2,3-dihydroxybenzoic acid; 8-OH-dG, 8-hydroxy-2′-deoxyguanosine; ACN, acrylonitrile; dG, 2′-deoxyguanosine; GSH, glutathione; GSH-Px, glutathione peroxidase; MDA, malondialdehyde; OTC, 2-oxothiazolidine-4-carboxylic acid; ROS, reactive oxygen species; SA, salicylic acid; SOD, superoxide dismutase

Journal Article.  6290 words. 

Subjects: Clinical Cytogenetics and Molecular Genetics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.