Journal Article

<i>In vitro</i> chemopreventive effects of plant polysaccharides (<i>Aloe barbadensis Miller</i>, <i>Lentinus edodes</i>, <i>Ganoderma lucidum</i> and <i>Coriolus versicolor</i>)

Hyung Sik Kim, Sam Kacew and Byung Mu Lee

in Carcinogenesis

Volume 20, issue 8, pages 1637-1640
Published in print August 1999 | ISSN: 0143-3334
Published online August 1999 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/20.8.1637
In vitro chemopreventive effects of plant polysaccharides (Aloe barbadensis Miller, Lentinus edodes, Ganoderma lucidum and Coriolus versicolor)

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A plant polysaccharide, Aloe gel extract, was reported to have an inhibitory effect on benzo[a]pyrene (B[a]P)–DNA adduct formation in vitro and in vivo. Hence, chemopreventive effects of plant polysaccharides [Aloe barbadensis Miller (APS), Lentinus edodes (LPS), Ganoderma lucidum (GPS) and Coriolus versicolor (CPS)] were compared using in vitro short-term screening methods associated with both initiation and promotion processes in carcinogenesis. In B[a]P–DNA adduct formation, APS (180 μg/ml) was the most effective in inhibition of B[a]P binding to DNA in mouse liver cells. Oxidative DNA damage (by 8-hydroxydeoxyguanosine) was significantly decreased by APS (180 μg/ml) and CPS (180 μg/ml). In induction of glutathione S-transferase activity, GPS was found to be the most effective among plant polysaccharides. In screening anti-tumor promoting effects, APS (180 μg/ml) significantly inhibited phorbol myristic acetate (PMA)-induced ornithine decarboxylase activity in Balb/3T3 cells. In addition, APS significantly inhibited PMA-induced tyrosine kinase activity in human leukemic cells. APS and CPS significantly inhibited superoxide anion formation. These results suggest that some plant polysaccharides produced both anti-genotoxic and anti-tumor promoting activities in in vitro models and, therefore, might be considered as potential agents for cancer chemoprevention.

Keywords: 8-OH-dG, 8-hydroxydeoxyguanosine; APS, Aloe barbadensis Miller polysaccharide; CPS, Coriolus versicolor polysaccharide; GPS, Ganoderma lucidum polysaccharide; GST, glutathione S-transferase; LPS, Lentinus edodes polysaccharide; ODC, ornithine decarboxylase; PMA, phorbol myristic acetate; TK, tyrosine kinase.

Journal Article.  2704 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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