Journal Article

Human DNA adducts of 1,3-butadiene, an important environmental carcinogen

Chunyan Zhao, Pavel Vodicka, Radim J. Šrám1 and Kari Hemminki

in Carcinogenesis

Volume 21, issue 1, pages 107-111
Published in print January 2000 | ISSN: 0143-3334
Published online January 2000 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/21.1.107
Human DNA adducts of 1,3-butadiene, an important environmental carcinogen

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The N-1-(2,3,4-trihydroxybutyl)adenine (N-1-THB-Ade) adducts induced by 1,3-butadiene (BD) were analysed from lymphocytes of 15 workers occupationally exposed to BD and 11 controls by 32P-post-labelling using HPLC with radioactivity detection. The difference in the adduct levels between the BD-exposed workers (4.5 ± 7.7 adducts/109 nucleotides) and the controls (0.8 ± 1.2 adducts/109 nucleotides) was statistically significant (Wilcoxon rank sum test, P = 0.038). This study shows for the first time BD-induced DNA adducts in humans and suggests that N-1-THB-Ade adducts may be used to biomonitor human exposure to BD.

Keywords: BD, 1,3-butadiene; EBD, epoxybutanediol; N-1-THB-Ade, N-1-(2,3,4-trihydroxybutyl)adenine; N6-THB-Ade, N6-(2,3,4-trihydroxybutyl)adenine.

Journal Article.  3557 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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