Journal Article

Decreased expression of <i>SPRR3</i> in Chinese human oesophageal cancer

Bao-Sheng Chen, Ming-Rong Wang, Yan Cai, Xin Xu, Zhi-Xiong Xu, Ya-Ling Han and Min Wu

in Carcinogenesis

Volume 21, issue 12, pages 2147-2150
Published in print December 2000 | ISSN: 0143-3334
Published online December 2000 | e-ISSN: 1460-2180 | DOI:
Decreased expression of SPRR3 in Chinese human oesophageal cancer

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cDNA fragments that were differentially expressed between human oesophageal carcinomas and matched normal adjacent mucosa were isolated using an improved mRNA differential display technique. One of them was identified as the 3′-untranslated region of SPRR3 and was homologous to the esophagin cDNA. Northern blot, dot blot and reverse transcription–polymerase chain reaction (RT–PCR) analyses revealed that SPRR3 expression was lost in three cell lines of oesophageal carcinoma and was dramatically decreased in 54 out of 57 primary oesophageal carcinomas compared with adjacent normal mucosa. Esophagin has been shown to be down-regulated in western oesophageal carcinomas. The data suggest that esophagin is probably the protein product of the gene SPRR3 and that altered mRNA expression of SPRR3/esophagin is a frequent event in the development of Chinese oesophageal cancer.

Keywords: RT–PCR, reverse transcription–polymerase chain reaction; SPRR, small proline-rich protein.

Journal Article.  2766 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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