Journal Article

Oxidative DNA damage in human lymphocytes: correlations with plasma levels of α-tocopherol and carotenoids

Franca Bianchini, Sölve Elmståhl, Carmen Martinez-Garciá, Anne-Linda van Kappel, Thierry Douki, Jean Cadet, Hiroshi Ohshima, Elio Riboli and Rudolf Kaaks

in Carcinogenesis

Volume 21, issue 2, pages 321-324
Published in print February 2000 | ISSN: 0143-3334
Published online February 2000 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/21.2.321
Oxidative DNA damage in human lymphocytes: correlations with plasma levels of α-tocopherol and carotenoids

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In order to investigate whether oxidative damage is associated with differences in antioxidant intake, we measured the levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) in lymphocytes and α-tocopherol and several carotenoids in plasma of women with different dietary habits. We found that women from Granada (Spain), a region with a typically Mediterranean diet, had significantly higher levels of 8-oxodGuo compared with Malmö (Sweden), a region with a Northern European dietary intake pattern (2.30 ± 0.78 versus 1.59 ± 1.01 8-oxodGuo/10–6 deoxyguanosine). Levels of plasma α-tocopherol and carotenoids were higher in Granada and these values were significantly positively correlated with levels of 8-oxodGuo. Our results do not support the hypothesis that a Mediterranean diet rich in α-tocopherol and carotenoids protects cells against oxidative DNA damage. It is possible, however, that consumption of foods other than fruits and vegetables, including fats, are responsible for the higher levels of 8-oxodGuo in Granada. Further studies are warranted to better elucidate the role of antioxidants in the modulation of oxidative stress in vivo.

Keywords: dG, deoxyguanosine; GC/MS, gas chromatography/mass spectrometry; 5-HMdUrd, 5-hydroxymethyl-2′-deoxyuridine; 8-oxodA, 8-oxo-7,8-dihydro-2′-deoxyadenosine; 8-oxodGuo, 8-oxo-7,8-dihydro-2′-deoxyguanosine.

Journal Article.  3215 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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