Journal Article

Biologic instability of pancreatic cancer xenografts in the nude mouse

Bruno M. Schmied, Alexis B. Ulrich, Hosei Matsuzaki, Tarek H. El-Metwally, Xianzhong Ding, Mirabella E. Fernandes, Thomas E. Adrian, William G. Chaney, Surinder K. Batra and Parviz M. Pour

in Carcinogenesis

Volume 21, issue 6, pages 1121-1127
Published in print June 2000 | ISSN: 0143-3334
Published online June 2000 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/21.6.1121
Biologic instability of pancreatic cancer xenografts in the nude mouse

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Tumor transplants into nude mice (NM) may reveal abnormal biological behavior compared with the original tumor. Despite this, human tumor xenografts in NM have been widely used to study the biology of tumors and to establish diagnostic and therapeutic modalities. Clearly, precise differences in the biology of a given tumor in human and in NM cannot be assessed. We compared the growth kinetics, differentiation pattern and karyotype of an anaplastic Syrian hamster pancreatic cancer cell line in NM and in allogenic hamsters. As with the original tumor, transplants in hamsters grew fast, were anaplastic and expressed markers related to tumor malignancy like galectin 3, TGF-α and its receptor EGFR at high levels. However, tumors in the NM were well-differentiated adenocarcinomas, grew slower, had increased apoptotic rate and had a high expression of differentiation markers such as blood group A antigen, DU-PAN-2, carbonic anhydrase II, TGF-β2 and mucin. Karyotypically, the tumors in the NM acquired additional chromosomal damage. Our results demonstrate significant differences in the morphology and biology of tumors grown in NM and the allogenic host, and call for caution in extrapolating data obtained from xenografts to primary cancer.

Keywords: BOP, N-nitrosobis(2-oxopropyl)amine; NM, nude mouse; SGH, Syrian golden hamster.

Journal Article.  5291 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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