Journal Article

NBP is the p53 homolog p63

Xiaoya Zeng, Yong Zhu and Hua Lu

in Carcinogenesis

Volume 22, issue 2, pages 215-219
Published in print February 2001 | ISSN: 0143-3334
Published online February 2001 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/22.2.215
NBP is the p53 homolog p63

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We previously identified a non-p53, p53-responsive DNA element (p53RE)-binding protein named NBP, functionally analogous to p53, from human cervical carcinoma Hela cells. Here we report a biochemical study demonstrating that this activity is the recently cloned p53 analog p63. NBP was purified through conventional and DNA affinity chromatography to apparent homogeneity with a prominent polypeptide migrating in between the 43 and 68 kDa positions on a SDS gel. This polypeptide immunoreacted with monoclonal anti-p63 but not anti-p53 or anti-p73 antibodies. Also, NBP co-purified with p63 through each step of fractionation, as detected with anti-p63 antibodies. DNA–protein complexes formed with purified NBP and p53RE-containing oligomers derived from the p21waf1 promoter were supershifted by anti-p63 but not anti-p53 antibodies. Thus, these results demonstrate that NBP is encoded by the p53 homolog p63 gene.

Keywords: EMSA, electrophoresis mobility shift assay; NBP, non-p53 p53RE-binding protein; p53CP, p53 competing protein; p53RE, p53-responsive DNA element.

Journal Article.  3716 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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